Journal: Molecules and Cells

Article Title: Tankyrase-1-mediated PARsylation directs TFEB partner switching to regulate selective Wnt target gene expression

doi: 10.1016/j.mocell.2026.100313

Figure Lengend Snippet: PARsylation of TFEB is crucial for its interaction with TCF-1/LEF-1 and Wnt-TFEB target gene expression. (A) TFEB with mutations at residues 237K and 274K is not PARsylated by TNKS1. (B, C) TFEB with mutations in the PARsylation sites does not bind to HA-TCF-1 (B) or HA-LEF-1 (C) in HEK293T cells. (D) The self-interaction of the TFEB mutant, which has mutations at PARsylation sites, was not reduced by the treatment of Wnt3a-CM. HEK293A cells were transfected with TFEB mutants carrying different tags and treated with Wnt3a-CM for 2 hours. (E) Treatment of HEK293A cells with Wnt3a-CM for 2 hours does not reduce the level of oligomer of TFEB PARsylation-defective mutants. (F) PARsylation of TFEB is necessary for Wnt-TFEB target gene expression. HEK293A cells were knocked down and transfected with the indicated constructs, then treated with Wnt3a-CM for 8 hours. The cells were then used for real-time PCR. Statistical analysis was performed using a 2-tailed unpaired t-test, showing exact P- values in each figure. Error bars indicate the standard deviation of triplicate measurements (technical replicates).

Article Snippet: Quantitative real-time PCR (qPCR) was conducted using THUNDERBIRD SYBR qPCR Mix (Toyobo, QPS-201) in accordance with the manufacturer’s instructions.

Techniques: Targeted Gene Expression, Mutagenesis, Transfection, Construct, Real-time Polymerase Chain Reaction, Standard Deviation

Journal: CNS Neuroscience & Therapeutics

Article Title: Aerobic Exercise Promotes Hippocampal Neurogenesis and Ameliorates Cognitive Dysfunction Induced by Unilateral Labyrinthectomy

doi: 10.1002/cns.70773

Figure Lengend Snippet: qRT‐PCR validation of gene expression changes related to cognitive function. (A–E, G, H, K, L) The Lyz2 (Ordinary one‐way ANOVA, F (2,27) = 102.0, p < 0.0001), Cd74 (Ordinary one‐way ANOVA, F (2,27) = 60.91, p < 0.0001), H2‐Eb1 (Ordinary one‐way ANOVA, F (2,27) = 136.9, p < 0.0001), Slc11a1 (Ordinary one‐way ANOVA, F (2,27) = 40.07, p < 0.0001), H2‐K1 (Ordinary one‐way ANOVA, F (2,27) = 90.56, p < 0.0001), Mid1 (Ordinary one‐way ANOVA, F (2,27) = 61.00, p < 0.0001), Gins2 (Ordinary one‐way ANOVA, F (2,27) = 65.38, p < 0.0001), Des (Ordinary one‐way ANOVA, F (2,27) = 26.82, p < 0.0001), Hba‐a1 (Ordinary one‐way ANOVA, F (2,27) = 35.63, p < 0.0001) genes were up‐regulated at 28d after UL and down‐regulated by aerobic exercise. (F, I, J) The Clec1a (Ordinary one‐way ANOVA, F (2,27) = 17.63, p = 0.0002), Cntn5 (Ordinary one‐way ANOVA, F (2,27) = 50.28, p < 0.0001), Glp2R (Ordinary one‐way ANOVA, F (2,27) = 58.78, p < 0.0001) genes were down‐regulated at 28d after UL and up‐regulated by aerobic exercise ( n = 10/group in qRT‐PCR).

Article Snippet: One microgram of RNA was reverse transcribed to complementary DNA (cDNA) using HiScript II Q RT SuperMix for quantitative reverse transcription PCR (qRT‐PCR) with a genomic DNA (gDNA) wiper ( R22301 , Vazyme, Nanjing, China). qRT‐PCR was performed on a BioRad CFX384 Real‐Time System using ChamQ SYBR qRT‐PCR Master Mix (Q311‐02, Vazyme, Nanjing, China) according to the manufacturer's protocol.

Techniques: Quantitative RT-PCR, Biomarker Discovery, Gene Expression